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The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

Briefly, FeFAB cells are the modified CRFK cells that express β-galactosidase under the control of the FFV LTR, which is trans-activated by the FFV Tas protein in relation to。

The molecular clone of feline foamy virus (pCF-7) and FeFAB cell for FeFAB assay were kind gifts from Dr. Martinfefab Lochelt, German Cancer Research Center, Germany.

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